Composite
Part:BBa_M36243:Design
Designed by: Tatyanna Dadabbo & Richard Hojel & Soraya Karimi Group: Stanford BIOE44 - S11 (2015-10-22)
Pro EP-B2 YebF Secretion Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 262
Illegal NheI site found at 285 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 414
Illegal BglII site found at 837
Illegal BglII site found at 1506
Illegal BamHI site found at 804 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1174
Illegal AgeI site found at 1258
Illegal AgeI site found at 1597 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1925
Design Notes
Testing whether Pro Ep-B2 protein is folded property and then successfully secreted via YebF.
Source
Pro EP-B2 DNA sequence and optimized E.Coli his-tag from "Computational Design of an α-Gliadin Peptidase" . YebF secretion system, RBS, terminator and rhamnose promoter from Gene Designer 2.0